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The fluorescence emission of GR undergoes a marked red-shift between 1 and 3 M GdnHCl (with a transition midpoint at ~2
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Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35)
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The favorable interactions between the protein and the glycans thus obtained were grouped in two categories, hydrogenbonding and hydrophobic
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Crystal structure of the disulfide bond-deficient Azurin mutant C3A/ C26A
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To prevent thermal- or scanner-drifts, images were recorded after a warmup phase of at least 2 h
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Malin became interested in a cometary globule called CG4 when photographs taken with the United Kingdom Schmidt Telescope in Epping showed that the object appeared dramatically different depending on whether it was viewed through a red or a blue filter
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These fractions were plotted versus Mg concentration and fitted to the cooperative binding equation F = F^sup max^([Mg]^sup n^/([Mg]^sup n^ + (K^sup Mg^^sub D^)^sup n^)), to yield an apparent dissociation constant K^sup Mg^^sub D^for Mg^sup 2+^, and an apparent cooperativity coefficient, n
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, greater LSE), an effect that largely replicates findings by Grella et al
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Mitral disease seen in association with fen-phen lesions included 3 cases with floppy MV, 2 cases with rheumatic heart disease, 1 case with calcific mitral annulus fibrosus, and 1 with superimposed infectious endocarditis (Figure 5)
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- Folding of phage P22 coat protein monomers: kinetic and thermodynamic properties
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. This inherent weakness of the Flory mean-field theory also demands extreme caution when extrapolating simulation or experimental results from peptides (10,12,43,58,101,102,129,138) to draw conclusions about denatured proteins
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. The ANS binding test extended to other proteins confirmed a widespread stressed condition of the globular structure in ice, indicating that oligomers' proteins are more susceptible to unfolding than monomers', presumably because the macromolecule can be further destabilized by subunit dissociation
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. TVs never really caught on as chalkboard replacements
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