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The "correctly folded" state of proteins: is it a metastable state? Angew
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- The transition curves of the amide I' hands were obtained according to a method described previously (28)
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., in photosynthesis) to folding processes that can take minutes or longer (1)
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. 6 and 7, it is also evident that a higher solder temperature improves wettability as the contact angle decreases for all fluxes
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. However, it was also found from the wetting balance test results (Figures 4 and 5) that the wettability of both solders is better on Cu substrates than on Ni substrates
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. Increased protein backbone conformational entropy upon hydrophobic ligand binding
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. Taken together, our results suggest that these hydrocarbon chain modifications do not play a significant role in the membrane binding stability of these proteins in accord with previous results (Philip and Scarlata, 2004)
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. The unfolding was triggered at t = 0 by a temperature-jump from 25°C to 75-95°C and was monitored by CD at 222 nm (Fig
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.01 nm in the complex form
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.This work was done by D
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., Personal communication, Sawgrass Technologies, Inc
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. But, after incubation at 20[degrees]C for 24 hours until the late stage, all six eggs identified as P
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. More recently, single-molecule FRET detection (Ha et al
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