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Changes in secondary structure of GR induced by GdnHCl were examined by CD measurements (Fig
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. How the dilatation symmetry of denatured states is broken under folding conditions will depend on a variety of factors including local biases for turns and short stretches of extended or helical conformations, the drive to sequester hydrophohic amino acids, and the achievement of specificity in side-chain packing (103)
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. MATERIALS AND METHODSPeptides and proteinsThe synthetic peplide corresponding to residues 86-105 of Gß^sub 1^ (NH^sub 2^-TTNKVHAIPLRSSWVMTCAY-CONH^sub 2^) and the corresponding control peptide that does not activate PLCß (Gß86-105(M101N)) were gifts from Dr
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- It is known that apomyoglobin is stable in its native state at neutral pH and 30 deg C
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