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Ligand-protein contacts (LPC) was derived with LPC software: automated analysis of interatomic contacts in proteins
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. For histogram simulations of the SL 4WJ at 10 mM Mg^sup 2+^, the state of the ribozyme system was allowed to stochastically switch between the extended undocked and quasi-docked states according to the assumed forward and reverse rate constants (see Fig
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- Higher protein concentrations lead to smaller red shifts upon thermal unfolding
2 corporate finance in international reader volume2 corporate finance in international reader volume
. 4), the compressibility factor being ?1 for mass fractions up to ~40% w/w
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. The transient nature of oligomeric intermediates of misfolded TTR that later mature into fibrillar aggregates makes them hard to study, and methods to study these species are sparse
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. The lower value for the Bis-ANS case is (at least) partially due to the shorter lifetime that will truncate the measurement since the rotational correlation time is considerably longer than the decay lifetime
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. Since a 1 wt% polymer concentration is used, the yield would be low
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. Normal horse serum solution (200 [micro]L), diluted 100-fold with PBS, was added to the wells to prevent any nonspecific reactions
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. Over time, both groups reported significant increases in the number of hours worked (p<
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