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The quality of the final products was characterized to be homogeneous on size exclusion chromatography and polyacrylamide gel electrophoresis in the presence and absence of SDS, and the purity of the protein was estimated to be above 98%
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. Conformational stability of globular proteins
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. For a given amino acid sequence, a complete simulation consists of 10^sup 7^ trial moves
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. CONCLUDING REMARKSGiven the severity of many diseases associated with protein misfolding and aggregation, including the amyloidoses and the transmissible spongiform encephalopathies, research in this area is intense
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. The high-temperature transition in apoA-2: DMPC disks (solid circles, Fig
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. Highly twisted fibrils, along with the species of negligible helicity, have been reported earlier for different insulin amyloid preparations (Nielsen et al
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. 13 kJ K^sup -1^ mol^sup -1^; see Fig
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. While this study showed an association between discrimination because of drug use and the health of illicit drug users, research on other stigmatized conditions such as HIV/AIDS and mental illness suggests that there are likely to be many other consequences of stigma and discrimination for drug users (48, 49)
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. 8453Number of obs = 13,680Number of groups = 1,401Obs per group: min = 8avg = 9
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- 6, it means that a chain will swell to make favorable contacts with the surrounding solvent and the chain is in a good solvent
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. In the pH-denaturant experiments followed either by CD or fluorescence (see below), the pH was measured before and after completion of experiments with an ullrathin Aldrich electrode in a Radiometer pH-meter (Copenhagen, Denmark)
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.2 M^sup -1^ and v = 0
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. In contrast to samples with pure buffer solutions, samples with NDSB do not show significant aggregation of thermal unfolded states
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. Since soman inhibition is known to stabilize the folded state, this implies that the soman-inhibited unfolded state
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