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fluorescent residues are involved in the self-association interface: and, 3), the value of 1 µM could be considered an upper estimation of the EI^sup sc^ self-dissociation constant
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. Immunol Cell Biol 2003; 81:289-296(51) Kirkham PA, Spooner G, Rahman I, et al
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. A peak close to zero FRET efficiency is always observed, and occurs in part due to photobleaching of the acceptor, as well as other processes that give rise to donor-only molecules (Deniz et al
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- And finally, thermal denaturalions were carried out to test for changes in protein stability upon binding to the effectors
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. The observation of well-resolved subpopulations in the FRET efficiency histogram indicates that the ribozyme can adopt two distinct conformations in solution
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