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Two intermediate states were populated at 2-3 M GdnHCl, as indicated by a combination of spectroscopic and chromatographic methods
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. They showed that the scaling of R^sub g^ with N follows a power law of the form R^sub g^ = R^sub o^N^sup 0
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- DSC was used to investigate whether the thermal denaturation of ACYI was reversible or not
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. That this is indeed the case is shown by comparing cavity statistics in the EV limit to values obtained for freely rotating chains
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. As the maximum size of a voxel that allows for intracellular spectroscopy in a Xenopus oocyte would be about (600 µm)^sup 3^, the same SNR as in our experiments could be achieved if the drug concentration were to be reduced by a factor of 27 (i
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. Macromolecular binding, however, prevents an exact quantification of the drug concentration-especially at or near the lower detection limit-in the different cellular compartments, which would be required for a detailed kinetic analysis of drug uptake
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. Hydrodynamic calculations on the CaM:SMLCK complex showed that its diffusion tensor is nearly axially symmetric with a (?^sup o^^sub m^)^sub app^ pattern for the ?-helices that is almost a mirror image of that in the CaM:SEF2-1mp complex, Fig
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.ACKNOWLEDGMENTSThis report was developed with support from the National Institute on Drug Abuse R01 DA017507-01 and the Center for Substance Abuse Treatment, Substance Abuse and Mental Health Services Administration (grant KD1-TI11433, and contract number 270-97-7011)
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