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- Our results show that dimeric EI^sup sc^ acquired a folded conformation at physiological pH: however, 8-anilino-1-naphthalene-sulfonic acid (ANS)-binding experiments, FTIR, and CD measurements suggested that the protein populated a molten-globule-like species at low pH
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. Folding and stability of El^sup sc^ in the absence of effectors Two unfolding mechanisms have been described in oligomeric proteins exposed to high temperatures or high concentrations of chemical denaturants at equilibrium (49-51): 1), dissociation followed by unfolding of the native or partially unfolded monomeric species; and 2), denaturation without the accumulation of the native dissociated species
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. Light scattering To estimate protein aggregation occurring upon thermal unfolding, the RF-1501 Fluorospectrometer was employed to measure elastic scattering at 500 nm
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. 3 shows the theoretical RT^sup n^^sub 1^ curves for a molecule with a correlation time, ?^sup o^^sub m^, of 10 ns with one internal motion, ?^sub if^, ranging between 20 ps and 6 ns, and squared order parameter, S^sup 2^^sub f^, ranging between 0
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. Mutational analysis of acylphosphatase suggests the importance of topology and contact order in protein folding
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