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and hence this wavelength was chosen as the emission wavelength for the isothermal denaturation melts
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. According to the polymeric paradigm (Fändrich and Dobson, 2002) of amyloidogenesis and the therein-implicated importance of backbone interactions, the more the native structure becomes distorted (and the protein backbone becomes exposed), the higher the chance that the protein steps onto the amyloidogenic pathway
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. These factors make the generality of findings from, for instance, hospitals, uncertain
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. For example, participants were asked if they used cocaine: never (coded as 0), once a month or less (coded as l), 2-3 days a month (coded as 2), about once a week (coded as 3), 2-3 days a week (coded as 4), 4-6 days a week (coded as 5) or everyday (coded as 6) over the past 2 months
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. Indeed, our other work with these data suggests that shuffling the roommate deck and pairing students who did not know one another prior to college entry may promote social understanding (Boisjoly, Duncan, Kremer, Levy, & Eccles, 2004)
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. In the Myrtle Beach case, federal prosecutors said in court (before being forced to retract their claim due to contrary testimony) that none of the clinic's 3,000 patients was "legitimate"; in other words, in their view every pain patient of all eight doctors was an addict
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- This leads us to further understanding of intermediate protein structures in the unfolding process
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. According to an established model of ?-condensation, thermodynamically unfavorable contact between DNA and PEG decreases the available free space for coil DNA in solution and DNA undergoes a collapse transition at some critical concentration of PEG
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. The importance of diffusive searches is unsurprising, since under biological conditions, all candidates for energetic interactions, including electrostatics, are locally screened to a few angstroms: much smaller than the dimensions over which sections of protein must move to find their native configurations
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. Large enthalpic (-259 ± 13 kJ µmol^sup -1^) and entropic changes (220 ± 13 kJ mol^sup -1^ at 310 K) upon binding were observed experimentally (Kwong et al
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.To avoid missing data and other complications of multiple roommates, we concentrated our analysis on the 990 individuals who were randomly assigned a single roommate
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