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The plateau level is preserved for DCVJ over 300 h, showing that this probe also binds to larger assemblies (spherical aggregates and protofibrils)
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. However, we note there was no significant effect of Advice/No-Advice, F(l, 14) = l
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- For Trp residues in proteins, it is not uncommon to find average lifetimes that are significantly longer than for free Trp
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. The stabilization of state C is probably due to an increase of its entropy, related to larger MSDs (in line with neutron scattering experiments on denatured ?-amylase, (51))
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. The enthalpic change upon gp120/CD4 complex formation, calculated as the difference in proteinprotein plus protein-solvent potential energy between the simulations of the complex and of the separate proteins (-127 kJ mol^sup -1^ at 300 K with a standard error, SE, of 11 kJ mol^sup -1^), is of the same order of magnitude as the experimentally determined value (-259 ± 13 kJ mol^sup -1^ at 310 K; Myszka et al
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