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Biophysical Characterization of the Enzyme I of the Streptomyces coelicolor Phosphoenolpyruvate:Sugar Phosphotransferase SystemHurtado-Gómez, EstefaníaABSTRACT The first protein in the bacterial phosphoenolpyruvate (PEP):sugar phosphotransferase system is the homodimeric 60-kDa enzyme I (EI), which autophosphorylates in the presence of PEP and Mg^sup 2+^
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. Although subcellular variations could not be determined with this whole-cell technique, it yielded spectra with increased resolution and sensitivity, since dipolar broadening and susceptibility variation within the sample are being reduced (30)
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. Single-pair FRET (spFRET) measurements can provide new insights into the details of RNA folding processes, by virtue of their enhanced abilities to report distributions and dynamics of molecular conformations (Ha et al, 1999; Zhuang et al
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. patients were nearly six times as likely as were their Canadian counterparts to have recently seen ads for most of the half-dozen prescription drugs that the study examined, reported a team of Canadian and U
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. Some patient advocates say drug warriors can't accept this reality because it undermines the logic of prohibition: If most people don't get hooked when exposed to the "hardest" of all categories of drugs, if patients' lives get dramatically better and they function perfectly well on doses that are supposed to incapacitate, stupefy, and derange, why is it so important for the government to protect us from these substances? From this point of view, the DEA must fight pain control because functional patients on high doses of opioids threaten its authority
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. Unfortunately, to answer comparatively simple questions about the effects of decision strategies (Greenberg, 1985) or shuffling (Gwynn, 1988) upon the outcomes in blackjack requires extremely lengthy and complex computer simulation
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. However, the thickness of the right ventricular papillary muscles was mildly increased, and microscopic sections of the right and left ventricles showed scattered myocyte hypertrophy
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. The market for weight loss drugs has fluctuated during the last 2 decades, spiking with approvals and dropping with high-profile recalls, said Laura Governale of FDA's division of surveillance, research, and communication support
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. Cost per prescription can be further broken down into two components: agesexMIX/Rx, the age/sex-specific mix of drug products selected per prescription within therapeutic categories, including changes in dosages selected; and PRICE/PILL, a price index for all products in each therapeutic category
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. between 1993 and 1998 was attributable to such referrals (Substance Abuse and Mental Health Services Administration 2001)
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- Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy
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. Neither of these methods, though, is voxel selective, nor do they employ ^sup 1^H, the dominant nucleus in cellular biochemistry
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. The FDA has identified a profile for individuals who may be at higher risk for sensitivity to sympathomimetic agents (Table I)
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