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The availability of an accurate theoretical framework for explaining scale-invariant properties of polymers in the EV limit has important ramifications for developing accurate theoretical descriptions for denatured proteins
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. A possible explanation for this large red shift during thermal unfolding, which is almost as large as those obtained with GndHCl-induced unfolding (see Table 2), may be given by the fact that the absent aggregation gives rise to only a weak burial of tryptophan residues
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. The result is an appropriately conservative testing of coefficients' statistical significance
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- It is impossible to capture the features of an ensemble, such as the creation of interior cavities, using a single conformation
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. the accessibility of atoms and residues, the backbone dihedral angles, and the hydrogen bond and electrostatic networks of the protein
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. The A-state also shows an increase in ß-sheet structure over time in a slow process suggestive of structure formation during self-assembly
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. However, the PLCß^sub 2^ N-terminal PH domain allows for full activation by Gß?-subunits (Wang et al
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. Third, in contrast to the reversible unfolding of lipid-free protein (indicated by the agreement between the heating and cooling CD curves in Fig
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. The polymer solution was poured into a dynamic light scattering (DLS) cell and cooled for 1 hour at 25°C
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