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- Ultra-violet fluorescence of actin
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. In this method, the fluorescence of donor and acceptor are simultaneously recorded using avalanche-photodiodes as individual molecules traverse the excitation volume in a confocal microscope, and the FRET efficiencies from many such single-molecule observations are plotted in the form of a histogram
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. This feature of FRET has been used extensively to study the structure and dynamics of biological molecules and assemblies, in ensemble as well as more recent single-molecule measurements (Selvin 2000; Klostermeier and Millar, 2001b; Weiss, 2000; Ha, 2001)
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