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There are two obvious differences between our approaches
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. Our work also leads to a direct solution to the reconciliation problem of Plaxco and coworkers (11,47,132)
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. With respect to tryptophan residues, five spectral classes of emission spectra have been identified which exhibit significantly different emission peak maxima
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. This is confirmed by EM showing that similar thermal treatment leads to a significantly larger increase in the diameter of the apoA-2-containing complexes (Fig
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. The prolonged 10-min incubation at 60°C promotes further elongation of the fibers even up to 2
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. The gp120/CD4 interaction is predominantly determined by the core of gp120: removal of the hypervariable loops corresponding to nearly 40% of the total sequence shows little effect on the thermodynamic properties of binding (Myszka et al
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. In line with the previous analysis of the functional elements, CD4-F43 shows a large intraresidue entropy reduction upon complexation (Fig
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- The dielectric spectra of protein aqueous solution have usually manifest at least three relaxation processes called delta, and gamma (Grant et al
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. This energy difference is used with the Metropolis criterion (61) to accept or reject the proposed move
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. Obes Res (In press)68
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.[FIGURES 1-2 OMITTED]On examination in our clinic, the patient indicated that she had mild bifrontal headache and nasal congestion
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