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. The joint position of the changes in the two adjacent lines was around 68°C, suggesting the appearance of an intermediate at ~68°C
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. For this final state we do not watch a significant difference between formerly thermal or GndHCl-induced unfolded states
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. Kinetic Stabilization and Fusion of Apolipoprotein A-2:DMPC Disks: Comparison with apoA-1 and apoC-1Jayaraman, ShobiniABSTRACTDenaturation studies of high-density lipoproteins (HDL) containing human apolipoprotein A-2 (apoA-2) and dimyristoyl phosphatidylcholine indicate kinetic stabilization
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- Cold denaturation-induced conformational changes in phosphoglycerate kinase from yeast
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. Once the first scan was completed, the samples were cooled down in situ to 283 K for 15 min and rescanned under the same experimental conditions to test for the reversibility of the denaturation reaction
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. On the other hand, at acidic pH, EI^sup sc^ lost its tertiary structure and solvent-exposed hydrophobic patches, as suggested by 1), the changes in the fluorescence intensity (Fig
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. This agrees with the DSC data from an identical sample indicating that disk reconstitution after heat denaturation takes more than 48 h to complete at 24°C (Fig
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. semifasciata and the extended time period (November-March) in which larvae were collected
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