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Once protein unfolding reached completion in the first jump, the sample was cooled and incubated at 25°C for 1000 s, which was sufficient for completion of the helical refolding (as indicated by flattening of the ?^sub 222^(t)) but not for the disk reconstitution (as indicated by DSC, EM, and gel electrophoresis) free home interest mortgage rate refinance shipping .
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Table 4Results of analyses of covariance testing for differences in slopesand elevations of annual absolute fecundity-age relation and annualrelative fecundity-age relation free home interest mortgage rate refinance shipping .
The peak position for the SL 3WJ remains in the low-FRET region of the histograms, independent of the Mg^sup 2+^ concentration, indicating that this ribozyme construct is incapable of folding to a compact structure (Fig free home interest mortgage rate refinance shipping .
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This medium is designated DMEM/ITS+/TGF-[beta]1 hereafter free home interest mortgage rate refinance shipping .
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