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3 B), and the second derivative spectra (Fig
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. 5-ns internal motion in the CaM:SEF2-1mp complex can be attributed to motions of the separate N- and C-terminal domains of CaM, because the free domains have an overall correlation time of ~3 ns
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. © 2005 by the Biophysical Society0006-3495/05/08/1227/10 $2
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. Such work is ongoing in our laboratories, and data from misfolded TTR oligomers recorded from site-specific fluorescence labeling of cysteine mutants have provided size distributions with average rotation correlation times of ~ 150 ns (K
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- The enzymes were dissolved in 30 mM Tris-HCl, pH 7
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. Since the major enthalpic contribution ?H* [congruent with] E^sub a^ to the free energy barrier ?G* = ?H*-T?S* for the disk denaturation may arise from the transient disruption of the predominantly hydrophobic protein-lipid interactions, similar values of E^sub a^ may reflect similar combined hydrophobic surfaces in the amphipathic ?-helices in apoA-1 and apoA-2 that are comprised of ~50 amino acids in the lipid-bound state of these proteins
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