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- Parameter A, which is a sensitive tool to reflect the position and shape of the Trp fluorescence spectrum, slightly decreased when the temperature increased to 64°C, and then an abrupt decrease was observed
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. Although the blank fluorescence for DCVJ is low, DCVJ in the presence of unfolded TTR shows even lower fluorescence than the blank, which could indicate a small quenching effect through binding
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. In line with previous postulates based on thermodynamic data (Kwong et al
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