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Between 2 and 3 M GdnHCl, two partially folded intermediate states possessing flexible tertiary structures (revealed by fluorescence data) but compact secondary structures (as indicated by circular dichroism measurements) were identified
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. Crystal structure of D-aminoacylase from Alcaligenes faecalis DA1
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. A scan of the protein structure database has shown that >75% of the proteins are glycosylated in nature
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. Although we observe a qualitative proportionality between smaller red shifts and a significant aggregation of thermal unfolded states (at least for BAA, BLA, and BSUA), we can not exclude other and maybe additional mechanisms responsible for smaller red shifts during thermal unfolding as compared to those obtained with GndHCl-induced unfolding
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. Figure 8 exhibits the dependency of wetting force and meniscus height on the perimeter (P) of the substrate
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. The fluorescent dyes 1-anilinonaphthalene-8-sulfonate (ANS), 4-4-bis-1-phenylamino-8-naphthalene sulfonate (Bis-ANS), 4-(dicyanovinyl)-julolidine (DCVJ), and thioflavin T (ThT) were employed in both static and kinetic assays to characterize these oligomeric and protofibrillar states using both steady-state and time-resolved fluorescence techniques
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. 5 mg/ml protein concentration was stored in the dark at 4°C and was used in four weeks, during which no protein degradation was detected
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. To start, mix ingredients should have maximum stability with regard to flavor, functionality and, in the case of protein-containing ingredients, protein stability
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. These include, but are not restricted to, native and near-native hydrophobic side-chain interactions
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. This work was done by D
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. Yet 44 out of the 49 defendants in this operation are of Indian descent, and in many cases their English falls far short of fluency
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. Table 3Self-Reported Depression Symptoms, Substance Use, and NationalNorms for Each GirlsBoysDepression (a) 12%9%National Normative Data (b) 8%7%Substance Use (c)29% 35%National Normative Data (d)24%(a) Percentage above average clinical cutoff (Kovacs, 1992)
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. Journal of Consulting and Clinical Psychology, 65, 130-140
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. This technique is called the "Pleasant Events Strategy" and is based on a behavioral theory of depression (17)
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. Chan's key rules--keep playing when you are winning, stop when you are losing, stay to an environment where you feel comfortable, take a vacation from poker when you need it (never try to win the money needed for a vacation from poker when you need one, that's a certain way to burnout), and discipline yourself to move back down to smaller tables when you're losing at big tables--are vital and should be taken to heart by all prospective professional poker players
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. Medication study visits were limited to 15 minutes to minimize the possibility that the study psychiatrist was providing other active psychotherapy ingredients to the treatment group
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. Editor's Note: Herbal "fen-phen" is not similar to the prescription drug combination of fenfluramine and phentermine
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. J Toxicol Clin Toxicol 1999; 37: 485-9
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- MATERIALS AND METHODS Materials Horse apomyoglobin was prepared by using Hapner's method (Hapner et al
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. Further work seems here necessary, and fluorescence probes exhibiting longer decay time is highly desirable for direct determination of the size distribution of the oligomer assemblies and protofibrils
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. ThT, on the other hand, requires a pH adjustment for its characteristic amyloid fluorescence signal (Fig
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. One may drop the assumption of local forces and allow the protein to "fall" toward the single native state down a "funnel" created by forces whose range permeate the entire volume
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. For the hairpin ribozyme, the best fit to the donor decay was obtained using a model of two Gaussian donor-acceptor distance distributions, with mean distances of 34-37 [Angstrom] and 75-78 [Angstrom] (N
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. Indeed, our other work with these data suggests that shuffling the roommate deck and pairing students who did not know one another prior to college entry may promote social understanding (Boisjoly, Duncan, Kremer, Levy, & Eccles, 2004)
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. We are also grateful to our early collaborators, Peter Fairweather, Rich Schwedtfeger, and Sam Detweiler, for their insights on this work
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