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To assay conformational preferences of highly denatured proteins, we quantify a variety of properties for EV-limit ensembles of 23 two-state proteins finance independent information provider yahoo .
5 to 4 µM, and only KI was used as the quencher finance independent information provider yahoo .
To enable characterization of different conformational states of TTR, we employed four different molecular probes: ANS, 4-4-bis-1-phenylamino-8-naphthalene sulfonate (Bis-ANS), 4-(dicyanovinyl)-julolidine (DCVJ), and thioflavin T (ThT) (Fig finance independent information provider yahoo .
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In those 2 studies, however, the staining was entirely cytoplasmic, whereas the staining observed in our cases was mainly located on the cell membrane, with only some marginal cytoplasmic immunoreactivity finance independent information provider yahoo .
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