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Yeast GR presents a quite blue-shifted fluorescence emission spectrum (maximal emission wavelength at 317 nm for the native protein; see Fig
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. The PTS involves a cascade of phosphoryl-transfer steps from phosphoenolpyruvate (PEP) via phosphointermediates of the general cytosolic non-sugar-specific phosphotransferases enzyme I (EI) and the histidine-phosphocarrier (HPr) to sugar-specific enzyme II permeases
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. The peak maxima range from ~308 nm for relatively nonpolar environments inside the protein globule to ?^sub max^-values of 350 nm which represent chromophores at the protein surface surrounded by polar water molecules (5,6)
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. Complexes of dimyristoyl phosphatidylcholine (DMPC; 95+% purity) with apoA-1 or apoA-2 were obtained by spontaneous reconstitution using protein/lipid ratio 1:4 mg/mg and overnight incubation at 24°C, followed by centrifugation at 13,000 rpm for 1
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. This feature is consistent with interconversion between compact and extended structures, which we estimate takes place on the 100-µs timescale using a simple model for the peak shape
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- After equilibration for 90 min in the presence of 3 M GdnHCl, major changes in fluorescence emission were observed
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