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Addition of 7 M GdnHCl completely abolished the CD signal at 222 nm, indicating extensive or complete unfolding of GR
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- All fluorescence spectra were corrected for background scattering as measured with pure buffer
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.Given ?^sub 1,2^ and a reasonable maximum of ?^sub ex^ (~6 ppm), a correlation plot of R^sub ex^ vs
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.5), which is necessary for the glutaraldehyde reaction
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [?], No changes in [?] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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