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. The absence of a long native baseline at this and other pH in EI^sup sc^ precluded any reliable conclusion on the pH independence of this value of T^sub m^
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. In conclusion, from examining azurin mutants of lower thermodynamic stability and greater internal flexibility than the WT, it was found that the perturbation of the native fold in the frozen state, evidenced by the capacity to bind ANS, is enhanced by the plasticity of the globular structure and correlates with the lability to freeze-thawing
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. Secreted DmAChE was purified to homogeneity using the following steps: 70% ammonium sulfate precipitation, dialysis, affinity chromatographies on a procainamide-Sepharose column and on nitrilotriacetic acid-nickel agarose (39)
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. Tjandra and co-workers (3) have shown, via ^sup 15^N-relaxation at multiple fields, that in free CaM the two domains undergo a slow "wobbling" motion on a timescale of ~3 ns, apparently not evident in target-bound CaM
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- Steady-state FRET methods were first used to monitor the docking of loops A and B during magnesium-induced folding (N
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.7(a) This prevalence is calculated using the total sample
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. Poor Black or Latino communities are the most frequent targets
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. As noted in our executive summary, to accomplish the infection, a hacker uses exploits described in the MS04-011 security update, dropping a script called ads
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