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- Under reducing conditions, the number of SH groups in urea-denatured yeast GR increases to six SH groups/monomer (Massey and Williams, 1965), indicating full exposure of all the Cys residues in the protein
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. There was no clear difference between N and A states in contrast to the 25% reduction of helix content and the 50% reduction of heat capacity change upon unfolding
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. In 2000, workers in Jeffery's laboratory transplanted an embryonic surface-fish lens into the optic cup, or eye socket, of a cave-fish embryo, and, conversely, transplanted a cave-fish lens into a surface-fish embryo [see illustration on page 46]
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. The drug we selected is an antitrypanosomal agent: diminazene aceturate (also referred to as berenil), the diminazene component of which is known to strongly bind to nucleic acid duplexes by forming hydrogen bonds (24)
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. The CaM:SEF2-1mp complex is found to be highly flexible with internal motions on the picosecond, nanosecond, and microsecond timescales
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.Dynamics on micro- to millisecond timescaleThree different approaches were used to determine which CaM residues in the CaM:SEF2-1mp complex are affected by conformational exchange
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. 5 B indicate that pressure does not promote the transition of PLCß to an activated conformation
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. Repetitive jumps to other temperatures yielded similar results
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.034ACKNOWLEDGMENTSSupport for this research was provided by grant DA11414 from the National Institute on Drug Abuse
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