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Abbreviations used: CD, circular dichroism; DTNB, 5,5'-dithiobis-(2-nitrobenzoic) acid; EDTA, ethylenediaminetetracetic acid; GdnHCl, guanidine hydrochloride; GR, yeast glutathione reductase; GSH, reduced glutathione; GSSG, oxidized glutathione; HEPES, n-(2-hydroxyethyl) piperazine-n'-(2-ethanesulfonic acid); HPLC, high performance liquid chromatography; MG, molten globule creative finance real estate .
By using a combination of fluorescence and circular dichroism spectroscopies, thiol group titration, and size-exclusion chromatography we have detected the existence of partially folded intermediate states in the unfolding of GR creative finance real estate .
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RESULTSDemographic characteristics of the sample are summarized in Table 1 creative finance real estate .
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