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Fluorescence studies of the conformational dynamics of parvalbumin in solution: lifetime and rotational motions of the single tryptophan residue
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. as in the case of the eyes of animals inhabiting dark caverns
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. 33× MR buffer (33 mM NaCl, 0
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. For k^sup -1^^sub ex^200 µs
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. We find that pressure does not increase the tendency of CPM-PLCß toward the fluorescence emission properties of the activated, Gß?-bound form (Fig
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. After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [?], No changes in [?] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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. 50%] = age at 50% maturity; [L
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. 880Note: Table made from line graph
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. Fleming T (editor): Physicians Desk Reference for Herbal Medicines, Ed 2
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. , Marti and Kochert 1996, Wink et al
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. They are asking, for example, whether:* PBMs are negotiating discounts on inflated prices* PBMs are receiving incentives from drug manufacturers to promote more expensive drugs* The rebates and discounts PBMs receive are being passed down to the plan sponsor and covered individualsState lawmakers are also concerned that the relationship weakens market competition
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- There was no clear difference between N and A states in contrast to the 25% reduction of helix content and the 50% reduction of heat capacity change upon unfolding
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.uk) by using the Needleman-Wunsch global alignment algorithm (with the following parameters: matrix: EBLOSUM62, gap penally value: 10
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. All fluorescence spectra were corrected for background scattering as measured with pure buffer
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. The measurement was repeated on five individual oocytes
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. A homodimeric peptide corresponding to the complete SEF2-1 basic sequence, henceforth called SEF2-1mp, was chosen as a good model system for NMR studies of the interactions between bHLH proteins and Ca^sup 2+^/CaM
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.DISCUSSIONThe dimeric CaM:SEF2-1mp complex shows unique dynamical behavior
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., K^sub p^ ~ 70 as opposed to 10 µM; Philip and Scarlata, 2004)
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. In such high-dimensional spaces, qualitatively new features may arise, such as energetically-flat domains that nonetheless are extremely difficult to escape from and so behave as kinetic traps
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.A complementary approach is to characterize the conformational distributions of freely diffusing RNA molecules using a single-molecule method referred to as diffusion spFRET (Deniz et al
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. Reexpression of the differentiated chondrocyte phenotype requires a plasma/serum factor, replaceable by TGF-[beta] and blocked by EGF
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.Third, as noted above, policy makers need to monitor carefully the negative effects that managed care may have on centers' involvement in interorganizational collaboration
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.([double dagger]) Dispersion index
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