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MATERIALS AND METHODS Materials Sodium dodecyl sulfate (SDS) was purchased from Sigma (St
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. Since no crystal structure of ACYI is available, all four of the components (A and S, I, II, and III) of Trp fluorescence defined previously (33,34) were included in the curve-fitting process
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. In the absence of internal motion or when there is only a small degree of fast (Rotational diffusion anisotropy and global orientationA cylinder-shaped model with a small degree of anisotropy has, according to Eq
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. The protein purity assessed by SDS gel electrophoresis was 95%
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. It is also important to ensure that the homogenizer valves fire maintained
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. All SL constructs show just a single peak in the FRET efficiency histograms, with no distinct docked peak, as expected (Fig
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. Eq (3), FF (3), Inv (3) Image Sun Tanning 5
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. ) For the most part, recent DEA activities have been aimed at curbing online sources for illegal drugs such as GHB, a tranquilizer, and other chemically similar substances, as well as using Web crawler and data mining technology to identify and prosecute illegal Internet pharmacies selling prescription drugs
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- On screen a strange world of shiny white globules slowly disintegrates as a woman's voice tells the story of a tragic affair
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.Backbone Dynamics of a Symmetric Calmodulin Dimer in Complex with the Calmodulin-Binding Domain of the Basic-Helix-Loop-Helix Transcription Factor SEF2-1/E2-2: A Highly Dynamic ComplexLarsson, GöranABSTRACTCalmodulin (CaM) interacts specifically as a dimer with some dimeric basic-Helix-Loop-Helix (bHLH) transcription factors via a novel high affinity binding mode
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. 3 A for comparison), and these bursts are analyzed to generate a FRET histogram (Fig
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