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A further increase in quenching efficiency was observed at 7 M GdnHCl (squares, K^sub SV^ = 40 M^sup -1^), indicating that even at 3 M GdnHCl the tryptophan residues of GR were still significantly protected from iodide quenching relative to the fully unfolded protein
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. Cell 2005; 123:321-334(12) Walsh GM, Sexton DW, Blaylock MG, et al
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. -Department of Electronic Engineering, City University of Hong Kong, Kowloon, Hong Kong
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. Global structure and dynamics of recombinant human Apolipoprotein CII in complex with SDS micelles: evident for increased helix mobility in the binding/activation site for lipoprotein lipase
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- coli GR: comparison of human and yeast GR yields ~68% of similarity and ~52% of sequence identity between the two proteins
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. Molecular graphics were created with PyMOL (38)
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. At basic pHs, the K^sub sv^s were similar, within the error, to those observed at pH 7
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. As a first step in this direction, we measured the thermal unfolding transition of ?-amylases in the presence of NDSB, which is known to be an effective agent to prevent aggregation of thermal unfolded proteins (32-34)
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. Proteins which do not present a single cooperative system
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. But, as we saw in the last section, some residues may provide dimensional reduction that significantly affects the overall rate via their nonnative interactions, without affecting the transition state itself
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. We have previously documented that high levels of depressive symptoms are associated with increased injection-related risk behaviors which place them at risk of HIV infection (8)
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