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In Protein Folding Handbook, Vol
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. ) was published in The New England Journal of Medicine on July 28, 2005
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- that the large amphipathic surface in the dimer interface could be exposed to solvent upon monomer formation; furthermore, there is a network of aspartic residues involved in salt bridges at the dimeric interface (Fig
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. Similar results have been observed in 1), the enzyme malate synthase G (54), a close structural analog of pyruvate dikinase and EIC (11), where binding to pyruvate does not cause large structural changes (55); and 2), the pyruvate dikinase from maize, where only small changes are observed in selected regions upon binding of PEP (56)
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. Two ml of a buffered BLG solution was dialyzed against buffered PEG solutions of known osmotic pressure (15,16) at 20°C for 24 h
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