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MATERIALS AND METHODS Materials Horse apomyoglobin was prepared by using Hapner's method (Hapner et al
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. N-linked oligosaccharides as outfitters for glycoprotein folding, form and function
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. The folding features of both domains are different
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. Solvent stabilization by salting-out ions is a consequence of a decrease in free energy of the native state, due to both entropic and enthalpic contributions; in particular, the effect on hydration water is expected to be mainly entropic, whereas the observed decrease in protein MSDs (44) indicates a tighter packing of the apolar residues in the core of the protein as suggested for hydrophobic stabilization by calorimetry experiments (59)
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. Piazza and Iacopini's light scattering study using BLG A (39) showed a minimum in the second virial coefficient at pH 4
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. Initiation of oligomerization was performed through addition of 100 mM NaCl from a 4 M stock solution to 0
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. We find that pressure does not increase the tendency of CPM-PLCß toward the fluorescence emission properties of the activated, Gß?-bound form (Fig
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. Furthermore, the evolutionary tailoring of nonnative interactions provides additional "design-space" within which a pathway to the folded state may be coded, but without compromising the stability of the final, native state
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. This suggests that two slightly different conformational spaces are sampled in the free and complex forms of the molecule, representing a situation of case A (Fig
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. RNA folding: models and perspectives
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. , 5 are unknown parameters estimated using ordinary least squares (OLS) and [[epsilon]
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. "A Generalized Index of Concentration
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. ([double dagger]) Dispersion index
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. Valentine JL, Kerrigan S
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- The latter refers to conditions under which the conformational ensemble can be modeled by ignoring all interactions except "two-body" repulsive (steric) interactions of the excluded volume (EV) kind
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. Stock solutions of ANS were prepared in water by using a molar extinction coefficient of 6
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. Taken together, these data suggest that the PSR gene product is not a true receptor for PS, although its potential contribution to efferocytosis in vivo by some other mechanism remains to be clarified
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.02 that were obtained following Meylan and Howard (28)
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.In contrast to reports on DNA binding with monocations, the compaction potentials of monocations determined here correlate well with biological assays that describe the compaction of chromosomes
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.DISCUSSIONIn this study, we have applied high pressure techniques to determine the volume change associated with PLCß-Gß? interactions on membrane surfaces and in buffer
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. Since pressure does not alter the surface charge or hydration of the membrane, electrostriction effects are not expected to play a significant role, and previous studies of the electrostatic component of the small peptide melittin correlate well with this idea (Teng and Scarlata, 1993)
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., 1998) and is released from mature plasma HDL at higher temperatures or higher denaturant concentrations than apoA-1 (Nichols et al
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., 2002), and the DSC data show only the lipid phase transition but lack the high-temperature peak because of the low unfolding enthalpy of this small protein
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. Exploring the folding landscape of a structured RNA
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. To eliminate these problems in the workplace, employers often incorporate drug policies that force potential employees to provide urine drug screens on condition of employment
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.-Considerable individual variation in the time course of anorectic agent use and the severity of fenphen valvulopathy was observed
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