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The amide I' region of native ACYI was dominated by bands from the ?-helix (1651 cm^sup -1^), ?-helix and/or 3 m helix (1660 cm^sup -1^), ß-sheet (1629, 1637, and 1682 cm^sup -1^), ß-turn (1668 and 1674 cm^sup -1^), and random coil (1644 cm^sup -1^)
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. In the case of azurin, to be consistent with the previous study (12), the protein concentration was raised to 2
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. ThT dramatically increases its fluorescence quantum yield when bound to amyloid fibrils; however, the mechanism behind this property is unknown
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. The increased temperature also appears to accelerate the elongation stage
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. Financial support from the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie and the COST D30 action "High Pressure Tuning of Biochemical Processes: Protein Dynamics and Aggregation" is gratefully acknowledged
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. In contrast, the evaluation of the spFRET data is modelindependent and the number of distinguishable conformers is directly revealed in the FRET efficiency histograms
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. Specifically, there was a significant, positive relationship between government revenues and IOR activity (p<
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