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The protein concentration used was 0
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. Predictions for the scaling of internal distances are important because they also allow us to make direct contact with measurements of internal distances in denatured proteins
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. Fluorescence characterization of different protein conformersThe A-state of TTR binds the classic hydrophobic dye ANS (Lai et al
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. This apparent K^sub d^, which reflects protein-protein associations on a confined membrane surface, is predicted to be ~2 orders of magnitude weaker when the proteins are free to associate in solution (see Runnels and Scarlata, 1999)
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. Clearly this coarse-grained level of model cannot pick up specific and discontinuously large shifts of the transition state, which in small proteins will often dominate particular cases
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- The above results from IR and intrinsic fluorescence spectroscopy strongly suggested that the thermal unfolding of ACYI was a step-wise process
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