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A 96 well plate was used and 40 µl of both the standards and samples were pipetted into each well; 250 µl of DMB solution was added to each and the absorbance at 595 nm was determined using a microplate reader (Bio-Rad Ltd, Hemel Hempstead, UK)
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. These effects can be minimized by adding small amounts of pysllium (mucilloid) (56)
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. In the final models for each of the two samples, a single coefficient was estimated for each conceptual relationship that was replicated across time intervals
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- N-Acyl-L-aromatic amino acid deacylase in animal tissues
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. In high concentrations of denaturants, proteins behave like polymers in a good solvent and ensembles for denatured proteins can be modeled by ignoring all interactions except excluded volume (EV) effects
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.In the case of Ni substrate, a binary Ni-Sn IMC was detected for Sn-Pb solder, whereas a ternary Cu-Ni-Sn IMC was found for Sn-Ag-Cu-Bi solder, as shown in Fig
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. This may reflect the large structural change and the crossing of the free and bound buildup curves (Fig
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