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Experiments were carried out at room temperature in 30 mM HEPES, at pH 7
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. Interestingly, the results from fluorescence and infrared (IR) spectroscopy clearly indicated that a pretransitional stage existed at temperatures from 50°C to 66°C
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. The free energy of unfolding calculated for rSBA and gSBA as per LEM model are 41
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. EI proteins are composed of two domains (6,7): a proteolytically inert N-terminal (EIN), and a proteolytically unstable C-terminal domain (EIC)
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. For example, milk-fat-globule-factor (MFG)-E8 and growth-arrest-specific-factor 6 (Gas6) are soluble bridging molecules that directly link PS on the apoptotic cell surface with their corresponding phagocyte receptors; [alpha]v[[beta]
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. The CaM binding site coincides with the basic DNA-binding sequence of the bHLH dimers (11,15,16,17)
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. In conclusion, the CaM:SEF2-1mp binding seems indeed distinct from other known protein:target binding modes
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. The approach to generate these species in solution opens up the possibility to probe the process by a wide number of biochemical and biophysical techniques to provide insight into the mechanism of protein misfolding and misassembly
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. , 2001b; Ahmad et al
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. Thus the printed fabrics had high flexural rigidities and, in many cases, unacceptable wet crockfastness ratings
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. Adding to concern, teens in some communities are engaging in dangerous trading sessions, where they gather whatever medications they can find--old prescriptions of their own, pills from their families' medicine cabinets--and swap them
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. " As an example, he cited a prescription for 1,600 pills a day
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. "Thanks to the range of options available, everyone in Medicare will be able to choose a prescription drug plan that addresses their individual concerns about cost, coverage and convenience," HHS Secretary Michael Leavitt said
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. Washington, DC: Administration for Children and Families, 2002
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. This medium is designated DMEM/ITS+/TGF-[beta]1 hereafter
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. It seems likely that AstraZeneca does not want to encourage others to follow my example
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. RELATED ARTICLE: Wyeth's Uphill ClimbWyeth's net revenue has been alternately battered and buoyed by a number of factors over the past decade, including gains from its divestiture of assorted consumer products, the recall of the diet drugs Redux and Pondimin in 1997, a national study critical of hormone products in 2002, and the success of its meningitis vaccine Prevnar
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. For PPA, which already exposes tryptophan residues to the protein surface in the folded state, a blue shift of the emission spectrum occurs upon thermal unfolding (see Fig
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