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Since K^sub SV^ equals the product of the bimolecular quenching rate constant (k^sub q^) and the excited-state lifetime of the fluorophore ([tau]), the value of K^sub SV^ obtained from steady-state fluorescence measurements is dependent on the average lifetime
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. The column was then washed extensively with PBS till the washings had A^sub 280^Isothermal guanidinium-hydrochloride-induced denaturation Equilibrium unfolding as a function of guanidinium hydrochloride (GdnCl) concentration was monitored by fluorescence spectroscopy
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. Identification of the N-terminal domain of enzyme I of the Excherichia coli phosphoenolpyruvate:sugar phosphotransferase system produced by proteolytic digestion
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. The resulting histogram can directly reveal conformational subpopulations that are averaged in conventional ensemble FRET measurements
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. PNT was influenced primarily by prior TX dose, with higher levels of TX dose preceding increases in PNT for both samples
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- More importantly, the position of the negative peak, which reflects the unfolding of the native structures, was centered at around 1628 cm^sup -1^ in the temperature range 30-66°C, then shifted to around 1639 cm^sup -1^ when the temperature increased from 66°C to 80°C, and finally remained at this wavenumber upon heating
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. Observations on the subcellular distribution of the ammonium ion in maize root tissue using in-vivo ^sup 14^N-nuclear magnetic resonance spectroscopy
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. 4) of each sample at a given temperature T was normalized to its elastic intensity at the lowest temperature (~20 K) for each detector, and the values so obtained were plotted in a semilogarithmic plot versus Q^sup 2^
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