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Our results show that the secondary structure of EI^sup sc^ was not substantially altered, as suggested by FTIR experiments, but quenching experiments and proteolysis patterns suggest that there were small conformational changes upon binding of PEP; in addition, the thermal stability of EI^sup sc^ was slightly modified when any effector was added
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. Our laboratory has previously characterized the affinity between PLCßs and their G-protein activators on model membrane surfaces using FRET (Runnels and Scarlata, 1999)
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. Posttransition pinguipedid juveniles were mainly collected at depths between 60 and 65 m, in both seasons sampled (summer and fall)
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. Those antibodies were named MT-1, MT-2, and MT-3
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- The thermal dependence of ACYI activity was measured by incubating the enzyme solutions at given temperatures for 30 min, and then the activity assay was performed by mixing the enzyme solutions and reaction buffers preheated at the same temperatures
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