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The appearance of the unusual exothermic peak and the lack of endothermic peak suggested that the unfolding of ACYI was accompanied by serious aggregation, which might be a nucleation-dependent process (36)
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. Of particular interest is the value of R^sub o^, which is related to the persistence length and also provides a measure of the goodness of the solvent because R^sup 3^^sub o^ quantifies the average volume per residue set aside by the chain for interactions with the surrounding solvent (38,41)
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