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Purification, characterization and possible function of ?-N-acylpeptide hydrolase from bovine liver
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. at both excitation wavelengths, showed a bell-shaped profile, with a maximum at neutral pH (Fig
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- Further, using the T4 DNA long-axis length distributions as shown in Fig
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.EXPERIMENTAL PROCEDURESProtein purificationTTR was expressed in BL21/DE3 cells transformed with the pmmHa plasmid
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. The lateral association is seen in the corresponding AFM images after 30 min at 60°C, but already after -20 min at 70°C
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