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Analysis of pH-dependent folding and stability of histidine point mutants allows characterization of the denatured slate and transition state for protein folding
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. Protein was extensively dialyzed against 2 L of the working buffer (5 mM HEPES buffer containing 10 mM NaCl and 2 mM ß-mercaptoethanol, pH 7) at 278 K
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. The N-terminal domain showed basically the same structure as the EIN^sup ec^ (8,9), since the alignments for homology modeling showed only minor deletions in loops between ?-helix I and ?-helix 2, ?-helix 2 and ?-helix 3, and ?-helix 3 and ?-helix 4 (Fig
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. The activation energy E^sub a^ was determined from the slope of these plots with the accuracy of 5-8 kcal/mol, which includes the fitting errors and the deviations among different data sets
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. Additionally, since the low E peak indicates a species with a longer inter-arm distance, we assign this peak to an extended (undocked) conformation
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. Participants were asked, "Have you ever been prevented from doing something, or been hassled or make to feel inferior because of any of the following?" Participants were offered a list to choose from including age, race, sex, sexual orientation, being poor, drug use, having been in jail or prison, or other
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. Since our sample of seventh graders already report higher levels of use than eighth graders nationwide, we can expect that by eighth grade these adolescents will report significantly higher substance use than will eighth graders nationwide
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. User Interface The user interface (Figure 2) comprised a single screen
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. Chapters discuss general poker concepts for the uninitiated, and the rules and favorable versus unfavorable situations for many poker variants including Limit Hold-em, Seven Card Stud, Eight Or Better Stud, Omaha High, Omaha Hi-Low, and No-Limit Hold-em
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. Never split 5s and 10s
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. "I think that the current management is making an effort to get the company headed in the right direction," says Ira Loss, executive vice president of the research firm Washington Analysis
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. "Importing from Canada also deprives patients of the prescription management, drug interaction review and counseling functions of licensed U
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. "The reasons behind each associations' opposition to the cross-border trade differ
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. In a statement, NABP said the surprising development forced it to take immediate and forceful action to "ensure the integrity of its testing program
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. Journal of Field Ornithology 67:499-506
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. Sexual size dimorphism in hawks and owls of North America
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. ([c] 2005 Book News, Inc
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. He begins with maps of the plague in the 17th century, and proceeds to Geographical Information System and mapping current diseases
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. Trends in compliance with treatment should be studied in relation to evidence-based clinical guidelines and adherence to treatment of appropriate duration and type should be encouraged through policy
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. And it bolsters the government's contention that private market competition will foster lower prices for seniors who participate in Part D
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. 3113Number of obs = 45,384Number of groups = 4,054Obs per group: min = 8avg = 11
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. Int J Addict 1983; 18(2):195-222
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. Pathology of drug abuse
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. With the exception of orlistat, which is not absorbed into the systemic circulation, obesity drugs should be avoided by women who are pregnant or breast-feeding
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. diethylpropion Available in generic and trade formulations
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. Easton, PA, Mack Publishing Company, 1990,18
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. Correspondence and requests for reprints should be addressed to Gary D
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. 5 percent increase in hourly wages (calculated as 100 * [exp(B) - 1]; see Hardy, 1993, pp
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. This backdoor, also known as Backdoor
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- The decrease in the content of Class I Trp fluorophores suggested that the intermediate had a loose tertiary structure, which allowed the penetration of water molecules into the hydrophohic interior
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. Since gSBA and rSBA both exist as tetramers with identical ligand binding specificities and macromolecular properties, the change of the mode of unfolding from a two-state process to a three-state process and the decrease of T^sub m^ by 26°C for the thermal unfolding of the protein of the kilter implicate the covalenlly linked glycans for the stability of gSBA
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. Protein dynamics: comparison of simulations with inelastic neutron scattering experiments
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. Competition of percolation and phase separation in a fluid of adhesive hard spheres
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. In fact there are large meanderlike areas, wherein fibrils are uniformly oriented
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. In this case the gutter-like structure survives a projection onto just two dimensions of folding space
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. The consequent mutual but general attraction of the helices for each other permits the final diffusive search for the native state to take place in a two-dimensional space, rather than the full five-dimensional search configuration space of all the diffusive degrees of freedom available to the protein once the secondary structure has formed
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. It is, however, not obvious to explain the slight, unexpected gain in entropy upon complexation for gp120 (Fig
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. The prints with the EVA toners were expected to be much more flexible than the prints made with IPP toners
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. We obtained similar results for the other six fabrics and the other curing temperatures
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. "Hospital Administrator Felt Needs for Interorganizational Cooperation and Actual Cooperative Outcomes by Their Hospitals
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. The results indicated a greater level of participation in gambling activities for intrinsic reasons than for extrinsic ones
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.A female jewelry shop worker in her late 20s also welcomed newopenings of foreign-run casinos, saying, ''It's good to have manychoices
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. Dealers are required to follow the casino's guidelines when playing; they cannot choose on their own regarding whether to hit or stand
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. Bull Johns Hopkins Hosp
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.2 Differentiation of plexiform lesions and cellular organizing or recanalizing thrombi can be difficult despite their prognostic implication as reported by the National Primary Pulmonary Hypertension Study Group sponsored by the National Heart, Lung and Blood Institute
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. Amphetamines also were used as weight loss agents, but their use often resulted in hypertension, addiction, and adverse myocardial events
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. Following the layout of a "funnel" interview, the conversation started with broad topics and then probed each response
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.40, we conducted further analyses of tolerance levels and found them to be acceptable (all above 0
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.Similar questions regarding marijuana use provide measures of any marijuana use in high school and monthly frequency of marijuana use "during the last 12 months
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. Randomized placebo-controlled trial of orlistat for weight loss and prevention of weight regain in obese patients
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