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4 × 10^sup 9^ M^sup -1^ s^sup -1^ (Eftink and Ghiron, 1981)
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. The integrated spectra were corrected by the fluorescence of each probe in the absence of protein and normalized to the recorded maximum intensity of each probe during the misfolding reaction
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. Most of the methanol was decanted, and the remaining methanol was allowed to evaporate
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. The fluorescence emission was collected using the same objective, separated from the excitation light using a dichroic filter (540 DRLP, Omega Optical, Brattleboro, VT), spatially filtered using a 100-µ pinhole, and separated into donor and acceptor components using a second dichroic filter (630 DRLP, Omega Optical)
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. 5 shows selected histograms from these measurements from 0
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. An individual's perception of support is thought to be a characteristic related not only to the actual availability of support but also to the criteria that individuals use in interpreting behavior as either supportive or nonsupportive (8)
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. By day seven, the pellets cultured in the DMEM/ITS+/TGF-[beta]1 group were noticeably larger than the corresponding pellets cultured in the DMEM/10%FCS group
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- Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells
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. The apparent dissociation constants for Mg^sup 2+^ (K^sub D^^sup Mg^) recovered from the fits are 2
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. Finally, there are other demographic changes that have not been accounted for in this study that will differ between BC, the U
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. It is important to note that the percentage of youth using substances increases with age (DHHS, 2001)
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. Change in functional and structural support (intake to year 2)Between Subject Mean effect at intake Mean (intake)(p-value) (year 2)Functional supportQuantity
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. A single-subject design was used with subjects serving as their own controls
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