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Folding of phage P22 coat protein monomers: kinetic and thermodynamic properties
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Protein concentration was calculated by using the extinction coefficients of Trp, Tyr, Cys, and Phe residues (24)
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Thermal denaturation experiments were also carried out by following the emission fluorescence of ANS either at 480 or 520 nm, after excitation at 360, 380, or 390 nm, at three different pH, 2
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For this final state we do not watch a significant difference between formerly thermal or GndHCl-induced unfolded states
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Quantitative estimation of protein binding site polarity
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Thus, in addition to the usual picosecond-timescale motions of varying contributions, a small amplitude nanosecond-timescale motion is present for all CaM residues in the CaM:SEF2-1mp complex
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The author is very grateful to Yuanjian Guo, Finly Philip, and Louisa Dowal for supplying many of the reagents
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33 K/min is sigmoidal and reaches a plateau at high temperatures, suggesting that the unfolding transition is complete at 99°C, yet the light-scattering curve recorded in the same experiment does not reach a plateau, and the signal continues to increase upon cooling from 99°C (Fig
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During most cruises, depths at which P
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8 64EH-04/9964[degrees]30'Pinguipes 1265
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Appending the free and complex trajectories of CD4 gives rise to a small stepwise increase of the entropy buildup of the backbone atoms (Fig
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t] is the number of days NSAID therapy dispensed per eligible senior Pharmacare beneficiary in month t (February 1993 [less than or equal to] t [less than or equal to]June 2001); data on the size of the BC senior population were obtained from Statistics Canada (Statistics Canada 2004)
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The FDA originally rejected BiDil, when it was submitted for approval in 1997 as a generic drug for treatment of heart failure, citing there was no conclusive evidence showing it was effective
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But the clock is ticking; under a rule approved this year by the Food and Drug Administration, CFCs no longer can be used in inhalers after 2008
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71Director hoursspent onlocal/statepolicymaking (log)0
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The client-side code downloads what some sources believe is the Padodor/Berbew backdoor Trojan, which records names and passwords linked to financial information and opens the machine to remote control
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To choose the other dealer, the player had to press any key on the keyboard within 1
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Special stains demonstrated a generally preserved elastic fiber layer, with some splaying at the base of plaques
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Glycemic control improved in diabetic patients taking orlistat
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98, and his percent of body fat was 36
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Item discrimination across subscales was good, with all subscales except "Readiness to refer" having at least 80 percent of items correlating significantly better with their own scale than with any other subscale
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I have found myself using it in many different situations: as a reference book, when a concise review of a topic is needed during the course of our sleep fellowship program, and as a tool for cross-checking information contained in other sleep textbooks
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In the end, we used the trimmed raw values for ease of interpretation and presentation
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The second PETS-A sample consisted of participants in an outpatient adolescent marijuana treatment experiment, entitled Cannabis Youth Treatment (CYT)
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The SAFE participants were recruited from the AIDS Linked to Intravenous Experiences (ALIVE) study, a natural history study of HIV infection in injection drug users in Baltimore (10)
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- Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells
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. Recently, Zhou (114) computed the functional form of x^sup 2^P(x) from EV simulations of proteins studied by Wilkins et al
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. It is insensitive to small variations in ^sup 15^N chemical shift anisotropy (CSA) (from -150 to -200 ppm) (22)
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. As noted in this case, cardiac valvular lesions were involved all four valves, with the tricuspid valve being most severely involved probably as a result of PPH, corresponding to the 40% of frequency in the tricuspid regurgitation observed in patients with PPH
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