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5 [Angstrom] at the average location of the center of mass
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. As a final remark we point out the close association found between ANS binding capacity (relative area, Table 2) and the peak wavelength of the fluorescence spectrum, ?^sub max^, the latter shifting further to the blue as the binding capacity increases
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. Residues 14, 19, 51, 55, 76, 92, and 118 show a relatively strong decrease in exchange rate upon change in temperature (f = 0
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. As reflected in our length-maturity logistic model, there was a decreasing trend in the percent maturity for female black rockfish in recreational landings from ODFW collections from 1992 through 2000 (Fig
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. 0098Corr (u_i, Xb) = -0
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- This suggests that proteins in the EV limit have an average ellipsoidal shape
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. This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure
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.Low-frequency modes have been shown to be affected by complex formation by MD simulations (49,50)
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. Hydrodynamic calculations can provide some indication as to the structural assignment of the internal motions identified from NMR relaxation experiments (see Supplementary Material)
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. Interestingly, all N-H^sup N^ vectors are also affected by an additional internal motion of ~2
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. Each standard concentration was used in triplicate
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